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Technologies to suit every project
Service Portfolio
MRM Proteomics Inc. offers a wide range of services to fit a wide variety of projects. The high quality of each of our services is driven by our leading-edge technologies and our demonstrated expertise.
Comprehensive Protein Structural Analysis by Combining MS-Based Approaches
Features & Benefits
- Multiple methods for lots of constraint data (crosslinking, surface medication, limited proteolysis, etc.)
- Recent high profile publications for protein structure determination
Applications
- Determine tertiary and quaternary structure for difficult-to-crystalize proteins
- Map binding sites and orientations
Consulting & Training
Features & Benefits
- Put our expertise to work for you
- Let us help you get the most out of your in-house proteomics facilities
- Attend a workshop or come to our site for one-on-one training. Site visits also available.
Applications
- Establish new proteomics workflows in house or implement a custom solution
- Improve existing protocols and analysis workflows
- Train new team members with best practices
Custom Solutions
Features & Benefits
- Let us use our expertise to develop, tailor or optimize an experimental protocol for your specific research question or project need
Applications
- Custom solutions for your specific experimental problem
- Solutions can be delivered as a service or may be transferred to the client for use in house (e.g., in kit format)
Development of Clinical Proteomics Assays using iMALDI
Features & Benefits
- Robust & easy to use
- Uses SIS peptides for reproducible, high precision quantitation
- Does not depend on antibodies for specificity
- Capable of isoform discrimination
- High-throughput & compatible with automation
- Compatible with equipment already installed in clinical labs
Applications
- Clinical applications
- Development of highly robust clinical tests to be used for improving diagnosis, risk stratification and treatment matching
- Development of assays for monitoring treatment response/resistance, toxicity, disease progression
High Resolution Protein Characterization by Hydrogen-Deuterium Exchange (Top/Middle-Down Analysis with & FT-ICR-MS of intact or partially proteolyzed protein)
Features & Benefits
- Higher-order structural characterization – reveals hydrogen bonding patterns and solvent exposure
- Close-to-single-residue resolution
- High sequence-coverage
- Reduced back-exchange
- Isoform-specific analysis
Applications
- Biomarker discovery
- Analyze secondary structure of proteins & antibodies <150 kDa
- Compare biosimilars to originator drugs
- Pinpoint conformational changes, bonding patterns, or binding sites
Metabolomics - Targeted
Features & Benefits
- >80 available UPLC-MRM/MS or UPLC-HRMS based assays for reliable quantitation of ~1000 metabolites and small molecules
- Convenient multiplexed methods to quantify metabolites in a given metabolic pathway or in a compound class manner
- Rigorously tested assays with established performance standards for sensitivity, linearity, reproducibility, reliability and stability
- Isotope-labeled internal standards for precise and accurate quantitation
Applications
- Biomarker screening, verification, and/or validation
- Analysis of key biochemical pathways
- Analysis of sugars, cofactors, phospholipids, bile acids, amino acids, fatty acids, carnitines, nucleotides, nucleosides, nucleobases, vitamins, steroids and phytohormones
- Highly multiplexed metabolite quantitation in a variety of complex biofluids and tissue types
Metabolomics - Untargeted
Features & Benefits
- Multiple UPLC-FTMS methods for untargeted metabolic profiling for discovery studies
- Identification and relative quantitation of known and unknown metabolites from one analysis
- Wide dynamic range for high and low abundance metabolites
- High resolution, high mass accuracy instrumentation for confident metabolite IDs and discrimination of closely related species
- Option to combine with MS/MS analysis to support high confidence IDs
- Established Quality Control procedures with SOPs
Applications
- Biomarker discovery
- Ideal for comparative studies: there is an unlimited number of samples that can be compared
- Lipidomic analysis
- Tailor extraction protocols to target different classes of small molecules
- Highly multiplexed metabolite quantitation in a variety of complex biofluids and tissue types
Peptide Synthesis
Features & Benefits
- fmoc peptide synthesis
- High purity products with CZE and AAA quality data
Applications
- Generate custom standards for proteomics analysis (natural abundance, heavy-labeled, or double-labeled isotopes)
Post-Translational Modification Analysis with FT-ICR-MS
Features & Benefits
- Characterize post-translational modifications (PTMs)
- Generate isoform-specific information
Applications
- Global glycosylation profiling
- Determine disulphide bonding patterns
Protein Biomarker Discovery by iTRAQ
Features & Benefits
- Untargeted proteomics approach for discovery studies
- Identification and relative quantitation of 1000s of peptides in one analysis
- Wide dynamic range for high and low abundance protein
- Detect post translational modifications can be detected
- Enhanced sensitivity with Orbitrap instrumentation
- Established Quality Control procedures with validated SOPs
Applications
- Biomarker discovery
- Ideal for comparative studies: up to 4 or 8 labels can be used for multiplexing experiments
Protein Characterization by Hydrogen-Deuterium Exchange (Bottom-up Analysis with & FT-ICR-MS of proteolyzed protein)
Features & Benefits
- Higher-order structural characterization
- Reveals hydrogen bonding patterns
- No size limitation on the proteins that can be analyzed
Applications
- Biomarker discovery
- Analyze secondary structure of proteins & antibodies <150 kDa
- Compare biosimilars to originator drugs
- Pinpoint conformational changes, bonding patterns, or binding sites
Protein ID & Sequencing
Features & Benefits
- Ultrahigh resolution intact mass measurement
- Protein/peptide sequencing – approaching single-residue resolution
Applications
- Determine exact mass of intact protein with extremely high accuracy
- Determine or confirm primary structure of peptides or proteins
Protein Quantitation by MRM-MS
Features & Benefits
- >500 available assays for accurate protein quantitation
- Quality MRM-MS technology you can trust: all assays are highly reproducible, rigorously tested for interferences, and characterized according to CPTAC guidelines
- Highly multiplexed panels for accurate quantitation of 100s of proteins from a single sample
- High purity paired heavy/light peptide standards for precise quantitation of every targeted protein
- Extremely high specificity and discrimination of protein isoforms
- No antibodies required
Applications
- Biomarker screening, verification, and/or validation
- Highly multiplexed protein quantitation in a variety of complex biofluids and tissue types
- Panels include med-low abundance proteins associated with cancer, CVD, and other diseases
Protein Quantitation of Low Abundance Analytes with Extreme Coverage by 2D-MRM-MS
Features & Benefits
- Enhanced sensitivity and multiplexing for deeper and more comprehensive protein quantitation
- Quantify up to 300 proteins from a single human plasma sample
- Extremely high specificity and discrimination of protein isoforms
Applications
- Biomarker screening and/or verification
- Highly multiplexed protein quantitation in a variety of complex biofluids and tissue types
- Maximum coverage of proteins in the sample
- Quantify low-abundance proteins without the need for an antibodies
Protein Quantitation of Selected Low Abundance Analytes by immuno-MRM
Features & Benefits
- Perform MRM-MS with an added enrichment/immunoprecipitation step to quantify very low abundance proteins
Applications
- Biomarker verification/validation
- Quantify selected low-abundance proteins using antibody-based enrichment, while maintaining extremely high specificity and discrimination of protein isoforms
Protein Structure Analysis using Crosslinking
Features & Benefits
- Characterize 3D protein structures (tertiary & quaternary) for difficult-to-crystalize proteins
- >40 custom crosslinking reagents for generating diverse constraint data
- In-house custom software for rapid and automated data analysis
Applications
- Analyze tertiary or quaternary structure of proteins
- Analyze protein folding or misfolding
- Determine protein complex assembly
- Study protein-protein interactions
- Generate constraint data for 3D structure modeling
Protein Structure Analysis using Limited Proteolysis
Features & Benefits
- Characterize 3D protein structures (tertiary & quaternary)
Applications
- Analyze tertiary or quaternary structure of proteins
- Analyze protein folding or misfolding
- Determine protein complex assembly
- Generate additional constraint data for 3D structure modeling
Protein Structure Analysis using Surface Modification
Features & Benefits
- Characterize 3D protein structures (tertiary & quaternary) for difficult-to-crystalize proteinsa
- >10 custom reagents for surface modification
Applications
- Analyze tertiary or quaternary structure of proteins
- Analyze protein folding or misfolding
- Determine protein complex assembly
- Generate additional constraint data for 3D structure modeling
Tissue Imaging of Proteins, Metabolites & Drugs by MALDI-FTICR-MS
Features & Benefits
- Visualize the Spatial Distribution of >600 Proteins, Peptides, Drugs & Metabolites
- Custom-developed MALDI matrix and coating techniques increase coverage and sensitivity
- FT-ICR-MS offers ultrahigh resolution for compound discrimination
Applications
- Analyze differential abundance of lipids and proteins across tissue samples
- Map the distribution of a drug in tissue
- Functional studies based on protein/metabolite abundance in different structures, compartments or tissue types.