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Technologies to suit every project

Service Portfolio

MRM Proteomics Inc. offers a wide range of services to fit a wide variety of projects. The high quality of each of our services is driven by our leading-edge technologies and our demonstrated expertise.

Development of Clinical Proteomics Assays using iMALDI

 

Features & Benefits

  • Robust & easy to use
  • Uses SIS peptides for reproducible, high precision quantitation
  • Does not depend on antibodies for specificity
  • Capable of isoform discrimination
  • High-throughput & compatible with automation
  • Compatible with equipment already installed in clinical labs

 

Applications

  • Clinical applications
  • Development of highly robust clinical tests to be used for improving diagnosis, risk stratification and treatment matching
  • Development of assays for monitoring treatment response/resistance, toxicity, disease progression
Protein Quantitation by MRM-MS

 

Features & Benefits

  • >500 available assays for accurate protein quantitation
  • Quality MRM-MS technology you can trust: all assays are highly reproducible, rigorously tested for interferences, and characterized according to CPTAC guidelines
  • Highly multiplexed panels for accurate quantitation of 100s of proteins from a single sample
  • High purity paired heavy/light peptide standards for precise quantitation of every targeted protein
  • Extremely high specificity and discrimination of protein isoforms
  • No antibodies required

 

Applications

  • Biomarker screening, verification, and/or validation
  • Highly multiplexed protein quantitation in a variety of complex biofluids and tissue types
  • Panels include med-low abundance proteins associated with cancer, CVD, and other diseases
Protein Quantitation of Low Abundance Analytes with Extreme Coverage by 2D-MRM-MS

 

Features & Benefits

  • Enhanced sensitivity and multiplexing for deeper and more comprehensive protein quantitation
  • Quantify up to 300 proteins from a single human plasma sample
  • Extremely high specificity and discrimination of protein isoforms

 

Applications

  • Biomarker screening and/or verification
  • Highly multiplexed protein quantitation in a variety of complex biofluids and tissue types
  • Maximum coverage of proteins in the sample
  • Quantify low-abundance proteins without the need for an antibodies
Protein Quantitation of Selected Low Abundance Analytes by immuno-MRM

 

Features & Benefits

  • Perform MRM-MS with an added enrichment/immunoprecipitation step to quantify very low abundance proteins

 

Applications

  • Biomarker verification/validation
  • Quantify selected low-abundance proteins using antibody-based enrichment, while maintaining extremely high specificity and discrimination of protein isoforms
Protein Biomarker Discovery by iTRAQ

 

Features & Benefits

  • Untargeted proteomics approach for discovery studies
  • Identification and relative quantitation of 1000s of peptides in one analysis
  • Wide dynamic range for high and low abundance protein
  • Detect post translational modifications can be detected
  • Enhanced sensitivity with Orbitrap instrumentation
  • Established Quality Control procedures with validated SOPs

 

Applications

  • Biomarker discovery
  • Ideal for comparative studies: up to 4 or 8 labels can be used for multiplexing experiments
Metabolite & Small Molecule Quantitation by MRM-MS

 

Features & Benefits

  • >400 available assays for accurate quantitation of metabolites
  • Convenient multiplexed methods to quantify metabolites from a given pathway or metabolite class
  • Rigorously tested assays with established performance standards for reproducibility, sensitivity, linear range, recovery, stability, etc.
  • Isotope-labeled internal standards for accurate and precise quantitation of every targeted metabolite

 

Applications

  • Biomarker screening, verification, and/or validation
  • Analysis of key biochemical pathways (e.g., Central carbon metabolism, tricarboxylic acid cycle, etc.)
  • Lipidomics analysis
  • Bile acids analysis (>100 different compounds, with custom-synthesized internal standards)
  • Highly multiplexed metabolite quantitation in a variety of complex biofluids and tissue types
Metabolomic Profiling

 

Features & Benefits

  • Untargeted metabolomics approach for discovery studies
  • Identification and relative quantitation of 1000s of metabolite features in one analysis
  • Wide dynamic range for high and low abundance metabolites
  • High resolution, high mass accuracy instrumentation for confident metabolite IDs and discrimination of closely related species
  • Option to combine with MS/MS analysis to support high confidence IDs
  • Established Quality Control procedures with validated SOPs

 

Applications

  • Biomarker discovery
  • Ideal for comparative studies: no limit to the number of samples that can be compared
  • Lipidomics analysis
  • Tailor extraction protocols to target different classes of small molecules
  • Highly multiplexed metabolite quantitation in a variety of complex biofluids and tissue types
High Resolution Protein Characterization by Hydrogen-Deuterium Exchange (Top/Middle-Down Analysis with & FT-ICR-MS of intact or partially proteolyzed protein)

 

Features & Benefits

  • Higher-order structural characterization – reveals hydrogen bonding patterns and solvent exposure
  • Close-to-single-residue resolution
  • High sequence-coverage
  • Reduced back-exchange
  • Isoform-specific analysis

 

Applications

  • Biomarker discovery
  • Analyze secondary structure of proteins & antibodies <150 kDa
  • Compare biosimilars to originator drugs
  • Pinpoint conformational changes, bonding patterns, or binding sites
Protein Characterization by Hydrogen-Deuterium Exchange (Bottom-up Analysis with & FT-ICR-MS of proteolyzed protein)

 

Features & Benefits

  • Higher-order structural characterization
  • Reveals hydrogen bonding patterns
  • No size limitation on the proteins that can be analyzed

 

Applications

  • Biomarker discovery
  • Analyze secondary structure of proteins & antibodies <150 kDa
  • Compare biosimilars to originator drugs
  • Pinpoint conformational changes, bonding patterns, or binding sites
Post-Translational Modification Analysis with FT-ICR-MS

 

Features & Benefits

  • Characterize post-translational modifications (PTMs)
  • Generate isoform-specific information

 

Applications

  • Global glycosylation profiling
  • Determine disulphide bonding patterns
Protein Structure Analysis using Crosslinking

 

Features & Benefits

  • Characterize 3D protein structures (tertiary & quaternary) for difficult-to-crystalize proteins
  • >40 custom crosslinking reagents for generating diverse constraint data
  • In-house custom software for rapid and automated data analysis

 

Applications

  • Analyze tertiary or quaternary structure of proteins
  • Analyze protein folding or misfolding
  • Determine protein complex assembly
  • Study protein-protein interactions
  • Generate constraint data for 3D structure modeling
Protein Structure Analysis using Surface Modification

 

Features & Benefits

  • Characterize 3D protein structures (tertiary & quaternary) for difficult-to-crystalize proteinsa
  • >10 custom reagents for surface modification

 

Applications

  • Analyze tertiary or quaternary structure of proteins
  • Analyze protein folding or misfolding
  • Determine protein complex assembly
  • Generate additional constraint data for 3D structure modeling
Protein Structure Analysis using Limited Proteolysis

 

Features & Benefits

  • Characterize 3D protein structures (tertiary & quaternary)

 

Applications

  • Analyze tertiary or quaternary structure of proteins
  • Analyze protein folding or misfolding
  • Determine protein complex assembly
  • Generate additional constraint data for 3D structure modeling
Comprehensive Protein Structural Analysis by Combining MS-Based Approaches

 

Features & Benefits

  • Multiple methods for lots of constraint data (crosslinking, surface medication, limited proteolysis, etc.)
  • Recent high profile publications for protein structure determination

 

Applications

  • Determine tertiary and quaternary structure for difficult-to-crystalize proteins
  • Map binding sites and orientations
Tissue Imaging of Proteins, Metabolites & Drugs by MALDI-FTICR-MS

 

Features & Benefits

  • Visualize the Spatial Distribution of >600 Proteins, Peptides, Drugs & Metabolites
  • Custom-developed MALDI matrix and coating techniques increase coverage and sensitivity
  • FT-ICR-MS offers ultrahigh resolution for compound discrimination

 

Applications

  • Analyze differential abundance of lipids and proteins across tissue samples
  • Map the distribution of a drug in tissue
  • Functional studies based on protein/metabolite abundance in different structures, compartments or tissue types.
Protein ID & Sequencing

 

Features & Benefits

  • Ultrahigh resolution intact mass measurement
  • Protein/peptide sequencing – approaching single-residue resolution

 

Applications

  • Determine exact mass of intact protein with extremely high accuracy
  • Determine or confirm primary structure of peptides or proteins
Peptide Synthesis

 

Features & Benefits

  • fmoc peptide synthesis
  • High purity products with CZE and AAA quality data

 

Applications

  • Generate custom standards for proteomics analysis (natural abundance, heavy-labeled, or double-labeled isotopes)
Custom Solutions

 

Features & Benefits

  • Let us use our expertise to develop, tailor or optimize an experimental protocol for your specific research question or project need

 

Applications

  • Custom solutions for your specific experimental problem
  • Solutions can be delivered as a service or may be transferred to the client for use in house (e.g., in kit format)
Consulting & Training

 

Features & Benefits

  • Put our expertise to work for you
  • Let us help you get the most out of your in-house proteomics facilities
  • Attend a workshop or come to our site for one-on-one training. Site visits also available.

 

Applications

  • Establish new proteomics workflows in house or implement a custom solution
  • Improve existing protocols and analysis workflows
  • Train new team members with best practices

Request more information on our services for Structural Characterization.

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