Bringing proteomics to the clinic
MRM Proteomics Inc. recognizes the promise of proteomics for clinical applications.
This vision has led us to partner on projects with PROOF Centre of Excellence and the Jain Foundation for developing proteomics-based biomarkers. The development of laboratory-developed tests (LDTs) will provide health-care providers with critical information to guide risk management, diagnosis and treatment plans. The value of biomarkers to the drug development pipeline is also well-established. Effective use of biomarkers in clinical trials can greatly expedite the process of bringing a drug to market. We are currently involved in a project with academic partners and AstraZeneca to develop and implement an iMALDI assay for patient stratification in clinical trials. See Press Releases for the details on these projects and others.
MRM Proteomics Inc. holds a license for patented iMALDI technology that enables rapid, robust, highly automated protein quantitation in complex samples. The iMALDI approach involves spiking protein digest from a biological sample with a stable isotope-labeled standard peptide matching the target, after which the endogenous peptides and standard peptides are co-captured on antibodies conjugated to magnetic beads.
The peptides are eluted from the antibodies on a MALDI plate by applying acidic MALDI matrix, followed by MALDI-MS analysis. The use of mass spectrometry (MS) with internal standards ensures highly precise quantitation. Furthermore, detection with MS provides added protection against interferences, since the assay does not depend exclusively on the antibody for specificity. This method is compatible with the Bruker Biotyper instrument, which has already been granted FDA approval for applications in microbiology.
iMALDI FEATURES & BENEFITS
Robust & easy to use
Uses SIS peptides for reproducible, high precision quantitation
Does not depend on antibodies for specificity
Capable of isoform discrimination
High-throughput & compatible with automation
Compatible with equipment already installed in clinical labs
APPLICATIONS & CURRENT PROJECTS
MRM Proteomics Inc is already playing a key role in the development and validation of technologies for clinical proteomics.
Project examples are shown below.
Popp R, Li H, Borchers CH
Expert review of proteomics
Zahedi RP, Parker CE, Borchers CH
Popp R, Basik M, Spatz A, Batist G, Zahedi RP, Borchers CH
Parker CE, Borchers CH
Popp R, Malmström D, Chambers AG, Lin D, Camenzind AG, van der Gugten JG, Holmes DT, Pugia M, Jaremek M, Cornett S, Suckau D, Borchers CH
Biochimica et Biophysica Acta
Camenzind AG, van der Gugten JG, Popp R, Holmes DT, Borchers CH
Mason DR, Reid JD, Camenzind AG, Holmes DT, Borchers CH