METABOLOMICS

Expert Metabolite & Small Molecule Services

The metabolome is an entire set of low-molecular weight molecules (typically <1,500 Da), commonly known as metabolites, within a biological system. Metabolomics, the study of the metabolome, aims to identify and quantify metabolites including their substrates, intermediates and end products in biosynthesis, metabolism, degradation and salvage; which concentrations are influenced by both genetic and environmental factors.  MRM Proteomics Inc. offers metabolomics services in two ways — targeted metabolomics and untargeted metabolomics.

TARGETED METABOLOMICS

MRM Proteomics offers 20 different services for targeted metabolomics. Our services allow for reliable quantitation of ~1000 metabolites & small molecules from key biochemical pathways or specific compound classes.

MRM Proteomics Inc. offers a variety of targeted  metabolomics and other small moclecule analysis services in a compound class  or pathway-specific manner for your metabolomics needs. Targeted metabolite services encompass the use of an array of custom-developed UPLC-MRM/MS methods, in combination of isotope dilution and/or chemical derivatizaiton techniques , to provide high-specificity, high-selectivity, and high-reproducibility detection and quantitation of hundreds of known metabolites in diverse biological samples such as body fluids, tissues, cells, culture media and plants. To make our services compatible for a variety of research demands, we have fully optimized sample preparation for several complex biofluids and tissue types in differing model organisms.  Precise and accurate quantitation of specific and targeted metabolites in human samples offers a promising route to disease diagnosis, therapeutic management and prognostic prediction in clinical applications.

SERVICE TYPES
Pathway Specific

 

 

Pathway Specific Targeted Metabolomics Services

 

PATHWAY

DESCRIPTION

VOLUME

One-carbon metabolism Quantification of metabolites in folate cycle, methione cycle, choline metabolism and transsulfuration pathway by LC-MS2x10(6) cells/50 mg tissues
Central carbon metabolismQuantification of glucose, sugar phosphates, CoAs, enzyme co-factors, TCA cycle carboxylic acids, phosphocarboxylic acids and 9 major nucleotides involved in Central Carbon Metabolism (CCM) by UPLC-MRM/MS2x10(6) cells/50 mg tissues
Amino acid metabolismQuantitation of amino acids, amines and selected metabolic intermediates 50 µL plasma/serum, 2x10(6) cells, 2x10(5) neurons or 50 mg tissues
Sphingolipid pathway-precursors, intermediates and end-product sphingolipids Quantification of sphingolipid biosynthesis intermediates, sphinganines, ceramides, sphingomyelins and selected gangliosides by UPLC-MRM/MS50 µL plasma/serum, 2x10(6) cells, 2x10(5) neurons or 50 mg tissues
Mevalonate pathway and isoprenoids/cholesterol synthesisDetection and quantitation of all known isoprenyl phosphate intermediates, isoprenoids (ubiquinones, dolichols, vitain K2 and squalene, etc.), cholesterol and other sterols by UPLC-MRM/MS and UPLC-FTMS50 mg tissues, 1x10(7) cells
Fatty acid metabolism - medium- to long-chain fatty acids and carnitines analysisSimultaneous quantification of medium- to long-chain fatty acids and acyl-carnitines by UPLC-MRM/MS50 µL plasma/serum, 2x10(6) cells, 2x10(5) neurons or 50 mg tissues
Purine-pyrimidine metabolism pathwayIdentification & quantification of nucleotides, nucleobases and nucleosides by UPLC-MRM/MS200 µL urine, 50 mg tissues, 1x10(7) cells
In vivo metabolites of vitamin co-metabolismQuantification of in vivo vitamins and metabolites of host-gut co-metabolism by UPLC-MRM/MS100 µL plasma/serum or 100 mg tissues
Class Specific

 

 

Class Specific Targeted Metabolomics Services

 

Class

Description

Volume

Bile acids analysis (I)Quantification of 51 unconjugated, taurine- and glycine-conjugated bile acids in human and rodent plasma/serum, bile, tissues, cells, cecal content, brain, neurons, urine and feces by UPLC-(-)MRM/MS25 µL plasma/serum, 1 µL bile, 200 µL urine, 2x10(6) cells or 20 mg tissues
Bile acids analysis (II)Quantitation of 51 compounds in bile acids analysis I and 16 bile acid sulfates and glucuronides in plasma/serum and urine in human and rodent plasma/serum, bile, tissues, cells, cecal content, brain, neurons, urine and feces by UPLC-(-)MRM/MS25 µL plasma/serum, 1 µL bile, 200 µL urine, 2x10(6) cells or 20 mg tissues
Bile acid synthesis precursors analysisQuantitation of 2 precursors of bile acid synthesis (7 α-OH chol-4-esten-3-one and 7α,12α-diOH chol-4-esten-3-one) in human and rodent plasma/serum, bile, tissues, cells, cecal content, brain, neurons, urine and feces by UPLC-(+)MRM/MS.50 µL plasma/serum, 2x10(6) cells or 50 mg tissues
Low-MW sugars analysisQuantification of ribose, xylose, ribulose, arabinose, rhamnose, xylulose, fucose, glucose, mannose, galactose, fructose, maltose, lactose, cellobiose by UPLC-MRM/MS50 µL plasma/serum, 2x10(6) cells or 50 mg tissues
Cardiolipin compounds analysisUPLC-(FT)MS/MS identification and UPLC-FTMS quantitation of cardiolipin compounds in cell, tissues, CSF and blood samples100 µL plasma/serum, 2x10(6) cells or 50 mg tissues
Aldehydes analysisQuantification of small molecule aldehydes (MDA, 4-HHE, 4-HNE, and C2 to C9 aldehydes) by chemical derivatization UPLC-MRM/MS100 µL plasma/serum and 0.5 mL urine
Steroid hormones analysisQuantification of about 30 steroid hormones by UPLC-MRM/MS100 µL plasma/serum, 100 mg tissues
2nd nucleotide messengers analysisQuantitation of 10 2nd nucleotide messengers2x10(6) cells or 50 mg tissues
Plant phytohormones analysisIdentification and quantification of 21 plant phytohormones by UPLC-MRM-MS100-300 mg, depending on tissue. Please inquire.
Short-chain (C2 to C6) fatty acids analysisQuantitation of 10 C2 to C6 short-chain fatty acids50 μL plasma/serum, 20 mg tissues
Full-profile fatty acids analysisQuantitation of short- to very long-chain (C2 to C26) saturated/unsaturated, even-/odd-carbon number, hydroxyl and di-carboxylic fatty acids 50 μL plasma/serum, 20 mg tissues
Free and acyl CoAs analysisQuantitation of free and C2 to C18 acyl-CoAs50 mg tissues, 1x10(7) cells

Pathway Specific Targeted Metabolomics Services

Class Specific Targeted Metabolomics Services

Features & Benefits

Isotope-labeled internal standards for precise and accurate quantitation

Rigorously tested assays with established performance standards for sensitivity, linearity, reproducibility, reliability and stability

Highly multiplexed metabolite quantitation in a variety of complex biofluids and tissue types — compatible with low sample volumes

Specific buffers are used & steps are taken to account for metabolites that are susceptible to side reactions

UNTARGETED METABOLOMICS

MRM Proteomics Inc. offers a variety of untargeted metabolomics services that are ideal for comparative studies — we will assess an unlimited number of samples.

Untargeted metabolomics is a discovery-based research approach focusing on detection and relative quantitation of thousands of known and unknown metabolites in the metabolome of a given biological system.  This technology is most advantageously used in comparative studies, to identify the changes in the metabolome among two or more experimental sample groups. MRM Proteomics Inc. uses UPLC coupled to high-resolution and high-mass accuracy MS instrumentation along with well established metabolome database searching  and quality control procedures to confidently determine the metabolite concentration changes. We also provide similar services for analysis of different lipid classes. As the field of metabolomics continues to mature, untargeted metabolomics is an important aspect of biomarker discovery in biological and clinical studies.

SERVICE TYPES

Service

Description

Volume

Untargeted metabolomics by UPLC-FT-MSUntargeted profiling and relative quantification of known and unknown metabolites by UPLC-MS with 4 LC-MS methods with (+) and (-) ESI detection 100 µL plasma/serum, 2x10(6) cells or 50 mg tissues
Untargeted metabolomics by UPLC-FT-MSUntargeted profiling and relative quantification of known and unknown metabolites by UPLC-MS with 2 LC-MS methods with (+) and (-) ESI detection 100 µL plasma/serum, 2x10(6) cells or 50 mg tissues
Untargeted lipidomics by UPLC-FTMSUntargeted profiling and relative quantification of lipids in plasma/serum, cells, tissues, plants and bacteria by UPLC-FTMS with (+) and (-) ESI detection100 µL plasma/serum, 2x10(6) cells or 50 mg tissues
Lipidomics by MALDI-MS tissue imaging Various lipids (phospholipids, glycerol lipids, fatty acids, acyl carnitines, etc.) in mammalian and plant tissues by MALDI-FTMS with (+) and (-) ion detectionWhole organs or tissues
Primary metabolites by MALDI-MS tissue imaging Major sugar phosphates, nucleotides, nucleotide-sugar conjugates Whole organs or tissues

Untargeted Metabolomics Services

Features & Benefits

High resolution, high mass accuracy instrumentation for confident metabolite IDs & discrimination of closely related species

Tailor extraction protocols to target different classes of small molecules

Established Quality Control procedures with SOPs

Wide dynamic range for high and low abundance metabolites

ASSOCIATED REFERENCES

Feb 2018

Liu T, Wang RX, Han J, Hao CZ, Qiu YL, Yan YY, Li LT, Wang NL, Gong JY, … , Wang JS

Jan 2018

Han J, Higgins R, Lim MD, Lin K, Yang J, Borchers CH

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Want to run metabolite panels on your own instruments? Consider our MetaboloMetrics™ kits.