FREQUENTLY ASKED QUESTIONS
About MRM Proteomics Inc.
Who is responsible for proteomics analysis at MRM Proteomics Inc.?
- MRM Proteomics Inc. draws on the expertise of >25 highly qualified scientific staff. Senior scientists lead the development of new methods and techniques in quantitative proteomics. Proteomics technicians then apply the validated methods high quality, cutting-edge analytical services. Together, this team has decades of experience in all aspects of assay design, peptide development, experimental optimization, mass spectrometry, and data analysis.
What instrumentation is used at MRM Proteomics Inc.?
MRM Proteomics Inc. customers gain access to the latest in state-of-the-art instrumentation including
a variety of mass spectrometers such as:
- Agilent Triple Quad LC-MS 6490/6495 MS
- Thermo Fisher LTQ Orbitrap Fusion MS
- Thermo Fisher LTQ Orbitrap Velos MS
- SCIEX QTRAP 4000 MS
- Bruker 12 Tesla FTICR-MS with a dual nano-electrospray and MALDI source
- Bruker Ultraflex III MALDI-TOF/TOF
- Bruker Biotyper MALDI
- Peptide Synthesizers
Does MRM Proteomics Inc. offer a certified lab environment (e.g., ISO, CLIA)?
MRM Proteomics Inc. is currently in the process of establishing a new certified lab environment in our new Montreal location.
Who are MRM Proteomics' partners?
- As a technology provider, MRM Proteomics has established its own labs in Victoria and Montreal. We continue to adopt the cutting-edge technologies developed by the UVic-Genome BC Proteomics Centre and McGill University’s Jewish General Hospital Proteomics Centre as well as other academic researchers and commercial partners . Dr. Christoph Borchers is our Chief Scientific Officer. Where required for complex projects, MRM Proteomics has on-board appropriate technology partners to maximize the value of the results.
- Cambridge Isotope Laboratories is a distributor of our kit line.
What is the difference between the various types of quality control kits?
PeptiQuant™ LC-MS Everyday Platform Performance Kits: includes a ready-to-use standard mix that can be quickly re-suspended and injected for routine instrument performance monitoring. The Everyday Platform Performance kit makes it easy to recognize retention time drift or changes in instrument performance.
PeptiQuant™ LC-MS Comprehensive Platform Performance Kits: Assesses instrument performance in detail by analyzing a complete standard calibration curve to ensure that your instrument is performing optimally across the entire concentration range. The Comprehensive Platform Kit makes it easy to recognize loss of sensitivity or changes in linear range.
PeptiQuant™ Workflow Performance Kits: Assesses the performance of your complete proteomics workflow, from sample preparation through to quantitation. Compare your generated protein concentrations to known reference values. The Workflow Performance Kit allows you to check the quality of your quantitation, and helps you to standardize your implementation, measure operator and instrument performance, and trouble-shoot your execution of MRM-MS workflows.
What is included in the kit boxes?
Our kits offer more than just standards, they are a complete solution for protein quantitation and quality control of proteomics workflows.
What proteins can be quantified with the PeptiQuant kits?
- Each kit has a specific set of proteins that it can quantify. To obtain a list of the specific protein targets for a given kit, please contact us.
Do the kits include low abundance proteins as targets?
- The MRM-MS methods used in the kits are free from depletion or enrichment steps and can typically quantify proteins that are found a med-high abundance. The most sensitive assays typically have lower limits of quantitation (LLoQs) in high picomolar range. To obtain a list of the specific protein targets for a given kit and their LLoQs, please visit the product page at Cambridge Isotope Laboratories. While MRM-Ms methods cannot match the sensitivity of some immunoassays, MRM-MS approaches offer the advantage of high multiplexing and extremely high specificity, including isoform discrimination. To quantify very low abundance proteins, we can offer specialized methods involving added depletion, enrichment, or prefractionation steps to facilitate quantitation of these targets. Pairing these methods with MS quantitation increases sensitivity while still achieving the extremely high specificity offered by MRM-MS approaches.