PeptiQuant™ Services

PEPTIQUANT™ PLUS

Expert Protein Quantitation by MRM-MS

PEPTIQUANT™ PROTEIN QUANTITATION

MRM Proteomics offers sample analysis using our high quality PeptiQuant™ Plus technology to quantify up to 300 proteins from a single sample.

Our PeptiQuant™ Plus assays employ a rigorous MRM-MS quantitation strategy that uses paired heavy/light peptide standards – a minimum of two synthetic peptides for every protein quantified.

The unlabeled “light” peptide is used to create the calibration curve, whereas the “heavy”’ SIS peptide is spiked into every sample at a fixed concentration. Using separate standards for calibration and normalization ensures robust and precise measurements, even at low concentrations.

FEATURES
  • Highly multiplexed panels for accurate quantitation of 100s of proteins from a single sample
  • High purity paired heavy/light peptide standards for precise quantitation of every targeted protein
  • Quality MRM-MS technology you can trust: all assays are highly reproducible, rigorously tested for intereferences, and characterized according to Tier II CPTAC guidelines
  • Panels include med-low abundance proteins associated with cancer, CVD, and other diseases
  • Compatible with a variety of sample types
  • Minimal sample volume (25-100 uL)
  • Suited to complex matrices analysis (plasma, CSF, urine etc.)
  • Extremely high specificity (not possible with antibody-based
    methods)
  • Discrimination of protein isoforms
  • Depletion or enrichment not required
  • No antibodies required

Protein target

Discovery

>350 proteins

Cancer

>75 proteins

Cardiovascular disease

>60 proteins

Apolipoproteins

>15 proteins

Sample type

HumanMouseBlood
CSF
Saliva
Other biofluids
Tissues
Cell lysates

Specialized capabilities

Low-volume samplesExtreme coverage (2D)Extra sensitivityAdvanced validation

Don’t see what you’re looking for?

Let us develop a custom assay for your specific application!

APPLICATIONS

Our highly-multiplexed panels are tailored for a wide variety of applications.

ASSOCIATED REFERENCES

2017

By LeBlanc, A., Michaud, S.A., Percy, A.J., Hardie, D.B., Yang, J., Sinclair, N.J., Proudfoot, J.I., Pistawka, A., Smith, D.S. and Borchers, C.H.

Multiplexed MRM-based Protein Quantitation Using Two Different Stable Isotope Labeled Peptides for Calibration.

Oct 01 2016

By Percy AJ, Hardie DB, Jardim A, Yang J, Elliott MH, Zhang S, Mohammed Y, Borchers CH

Multiplexed panel of precisely quantified salivary proteins for biomarker assessment

Nov 01 2016

By Percy AJ, Michaud SA, Jardim A, Sinclair NJ, Zhang S, Mohammed Y, Palmer AL, Hardie DB, Yang J, LeBlanc AM, Borchers CH.

Multiplexed MRM?based assays for the quantitation of proteins in mouse plasma and heart tissue.

2015

By Hirtz, Christophe, Jérôme Vialaret, Nora Nowak, Audrey Gabelle, Dominique Deville de Périère, and Sylvain Lehmann

Absolute quantification of 35 plasma biomarkers in human saliva using targeted MS.

Jun 30 2014

By Percy AJ, Yang J, Chambers AG, Simon R, Hardie DB, Borchers CH..

Multiplexed MRM with internal standards for cerebrospinal fluid candidate protein biomarker quantitation.

Dec 01 2013

By Percy AJ, Byrns S, Chambers AG,
Borchers CH.

Targeted quantitation of CVD-linked plasma proteins for biomarker verification and validation.

Targeted mass spectrometry assays were characterized at the UVic-Genome BC Proteomics Centre according to the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) assay characterization guidelines (https://assays.cancer.gov/guidance-document). Initial characterization data (produced in a human plasma matrix), can be viewed on the CPTAC Assay Portal (https://assays.cancer.gov). Note: Specific kits may use a different type of matrix from the human plasma that was used to generate the CPTAC fit-for-purpose characterization data, and thus assay performance from this kit may vary from that reported on the CPTAC Assay Portal. The CPTAC assay characterization guidance documents were developed as a direct outcome of a workshop (Carr et al., Mol Cell Proteomics 2014; PMID 24443746) sponsored by the CPTAC program.

Looking for even wider coverage for biomarker discovery? Consider an iTRAQ experiment.

Want to run PeptiQuant™ panels on your own instruments? Consider our PeptiQuant™ kits.

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